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Characterization of benzoate degradation by newly isolated bacterium Pseudomonas sp. XP-M2

Paper ID Volume ID Publish Year Pages File Format Full-Text
4235 216 2009 4 PDF Available
Title
Characterization of benzoate degradation by newly isolated bacterium Pseudomonas sp. XP-M2
Abstract

An effective benzoate-degradation strain XP-M2 belonging to the genus Pseudomonas, was newly isolated from aromatic compounds contaminated soils. The growing cells of strain XP-M2 completely degraded 10 g/L benzoate within 12 h, and the degradation efficiency was found to be higher than other reports. Catechol and cis,cis-muconic acid as the metabolic intermediates were identified by gas chromatography–high-resolution mass spectrometry, and the catA gene encoding catechol 1,2-dioxygenase in this strain was cloned and expressed in Escherichia coli, which indicated that benzoate degradation by Pseudomonas sp. XP-M2 was via the ortho-cleavage pathway. In addition, the Andrews model was used to describe the functional relationship between specific growth rate and benzoate concentrations with kinetics parameters: KS = 2.47 g/L, KI = 3.81 g/L.

Keywords
Benzoate; Kinetics; Andrews model; Microbial; Bioremediation; Waste treatment
First Page Preview
Characterization of benzoate degradation by newly isolated bacterium Pseudomonas sp. XP-M2
Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 46, Issue 1, 1 September 2009, Pages 79–82
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering