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ERK-like MAPK signaling and cytochrome c response to oleic acid in two-liquid-phase suspension cultures of Taxus cuspidata

Paper ID Volume ID Publish Year Pages File Format Full-Text
4248 217 2008 6 PDF Available
Title
ERK-like MAPK signaling and cytochrome c response to oleic acid in two-liquid-phase suspension cultures of Taxus cuspidata
Abstract

To elucidate the underlying signal mechanisms of Taxus cuspidata in response to oleic acid (OA) in two-liquid-phase suspension cultures (TLPSCs), the mitogen-activated protein kinase (MAPK) activation and release of cytochrome c (CytC) from mitochondria in TLPSC T. cuspidata were investigated with Western blot. The results showed that a long-term treatment with 4% (v/v) OA caused apoptosis and release of CytC from mitochondria and inhibited the activation of extracellular signal-regulated kinase-like (ERK-like) MAPK (approximately 46 kDa) in TLPSC of T. cuspidata, but a short-term treatment significantly increased the ERK-like MAPK activity. These findings suggest that the coordinate expression of p-ERK and CytC is fundamentally involved in cell survival or death in TLPSC T. cuspidata. Further investigation confirmed that anthracene-9-carboxylate (an ion-channel blocker) and suramin (a G-protein inhibitor) markedly blocked the ERK-like MAPK activity, inhibited DNA ladder formation and O2− burst and reduced Taxol production. Although diphenyliodonium (an inhibitor of O2− formation) successfully blocked DNA ladder formation and decreased Taxol production, it did not significantly affect the ERK-like MAPK activity, indicating that the ERK-like MAPK activation should depend on the OA-stimulated G-protein and ion-channel activity, but not on the oxidative burst during the apoptosis and Taxol production in TLPSC T. cuspidata cells.

Keywords
Two-liquid-phase; Mitogen-activated protein kinase; Cytochrome c; Oleic acid; Taxus cuspidata
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ERK-like MAPK signaling and cytochrome c response to oleic acid in two-liquid-phase suspension cultures of Taxus cuspidata
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 41, Issue 3, 1 October 2008, Pages 274–279
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us