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Enzymatic reactions inside a microreactor with a mesoporous silica catalyst support layer

Paper ID Volume ID Publish Year Pages File Format Full-Text
42526 45929 2009 5 PDF Available
Title
Enzymatic reactions inside a microreactor with a mesoporous silica catalyst support layer
Abstract

A microreactor containing mesoporous silica thin film that we recently developed [Appl. Catal. A: Gen. 342 (2008) 107–112] was employed to realize a continuous enzymatic reaction. The mesoporous silica thin film served as a catalyst support layer for immobilizing lipase without complex chemical modification. The immobilized lipase showed good enzymatic activity and stability during a 24 h continuous ester hydrolysis reaction. The relation between the product and reactant concentrations indicated that the hydrolysis reaction with immobilized lipase follows Michaelis–Menten kinetics. The activity of the immobilized lipase was evaluated using a mathematical model and was compared with that of unimmobilized lipase in a batch experiment. The obtained turnover number of the immobilized lipase was almost the same as that of the free lipase in the batch experiment, while the Michaelis constant of the immobilized lipase was greater than that in the batch experiment by a factor of 3. A mathematical model was also employed to interpret the results of microreactor experiments performed under various conditions.

Graphical abstractA microreactor containing mesoporous silica thin film was employed to realize a continuous enzymatic reaction. The mesoporous silica film served as a catalyst support layer which immobilized the enzyme without complex chemical modification. The immobilized enzyme showed good stability during continuous reactions. A design equation for the microreactor model was introduced to interpret the activity of lipase in the hydrolysis reaction.Figure optionsDownload full-size imageDownload as PowerPoint slide

Keywords
Immobilization; Mesoporous silica; Catalyst support; Lipase; Hydrolysis
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Publisher
Database: Elsevier - ScienceDirect
Journal: Applied Catalysis A: General - Volume 359, Issues 1–2, 15 May 2009, Pages 108–112
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Catalysis
Get Full-Text Now
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Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
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Any Questions? feel free to contact us