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Enhanced degradation of caffeine by immobilized cells of Pseudomonas sp. in agar–agar matrix using statistical approach

Paper ID Volume ID Publish Year Pages File Format Full-Text
4261 218 2009 6 PDF Available
Title
Enhanced degradation of caffeine by immobilized cells of Pseudomonas sp. in agar–agar matrix using statistical approach
Abstract

Previously, we isolated caffeine degrading Pseudomonas strain from soil of coffee plantation area, which could utilize caffeine as sole carbon and nitrogen source and could tolerate caffeine up to 20 g/L. In this study, caffeine degradation by immobilized cells of this strain was investigated. Various matrices were considered and agar–agar was chosen based on degradation rate (0.08 g/(L h)), bead stability and reusability. Further, immobilization parameters, viz., bead size (mm), agar–agar concentration % (w/v) and cell concentration (g/L) were optimized using central composite design. The optimal conditions of cell concentration, agar–agar concentration and bead size were 7.8 g/L, 5% (w/v) and 6.2 mm. Under optimal conditions, caffeine degradation rate was found to 0.15 g/(L h), which closely agrees with the model predicted values. This is the first report on caffeine degradation at high concentrations (10 g/L) by immobilized cells of Pseudomonas sp. Immobilization efficiency was 80%. Damköhler number is very much higher than 1, suggesting that mass transfer is the rate limiting process.

Keywords
Biodegradation; Immobilization; Immobilized cells; Kinetic parameters; Submerged culture; Diffusion reaction
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 44, Issues 2–3, 15 May 2009, Pages 136–141
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us