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Influence of the operating conditions on lipase-catalysed synthesis of ricinoleic acid estolides in solvent-free systems

Paper ID Volume ID Publish Year Pages File Format Full-Text
4271 218 2009 6 PDF Available
Title
Influence of the operating conditions on lipase-catalysed synthesis of ricinoleic acid estolides in solvent-free systems
Abstract

In this work, the synthesis of ricinoleic acid estolides, also named polyricinoleic acid (PR), in two different solvent-free reaction systems, catalysed by immobilised Candida rugosa lipase is described. First, the esterification was performed in an open air jacketed batch reactor and the influence of amount of enzyme, temperature and initial water content was tested. The best results were obtained when 5 g of soaked immobilised derivative was used as biocatalyst, and the reaction was carried out at 40 °C. It was observed that environmental relative humidity plays an important role in the enzymatic synthesis of ricinoleic acid estolides and, given that this parameter takes on a wide range of values depending on the season, it is considered the main cause of the poor reproducibility achieved in the open air reactor. To solve this problem, the ricinoleic acid estolides were synthesised under controlled atmosphere in a vacuum reactor with dry air intake. The optimum drying time of 7 h was selected. In these conditions, PR with an acid value of 57.5 mg KOH/g was synthesised in 48 h of reaction and the results were entirely reproducible.

Keywords
Enzyme; Immobilised; Bioreactor systems; Stirred tank; Ricinoleic acid estolide; Solvent-free
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Influence of the operating conditions on lipase-catalysed synthesis of ricinoleic acid estolides in solvent-free systems
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 44, Issues 2–3, 15 May 2009, Pages 214–219
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us