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Biodiesel production from canola oil by using lipase immobilized onto hydrophobic microporous styrene–divinylbenzene copolymer

Paper ID Volume ID Publish Year Pages File Format Full-Text
4272 218 2009 6 PDF Available
Title
Biodiesel production from canola oil by using lipase immobilized onto hydrophobic microporous styrene–divinylbenzene copolymer
Abstract

In this study, the methyl esters of the long chain fatty acids (biodiesel) were synthesized by methanolysis of canola oil by immobilized lipase. Lipase from Thermomyces lanuginosus was immobilized by both physical adsorption and covalent attachment onto polyglutaraldehyde activated styrene–divinylbenzene (STY–DVB) copolymer, which is synthesized by using high internal phase emulsion (polyHIPE). Two different STY–DVB copolymers were evaluated: STY–DVB copolymer and STY–DVB copolymer containing polyglutaraldehyde (STY–DVB–PGA). Lipase from T. lanuginosus was immobilized with 60% and 85% yield on the hydrophobic microporous STY–DVB and STY–DVB–PGA copolymer, respectively. Biodiesel production using the latter lipase preparation was realized by a three-step addition of methanol to avoid strong substrate inhibition. Under the optimized conditions, the maximum biodiesel yield was 97% at 50 °C in 24 h reaction. The immobilized enzyme retained its activity during the 10 repeated batch reactions.

Keywords
Biofuels; Immobilized; Biocatalyst preparation; Enzyme; Styrene–divinylbenzene copolymer; Polyglutaraldehyde
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Biodiesel production from canola oil by using lipase immobilized onto hydrophobic microporous styrene–divinylbenzene copolymer
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 44, Issues 2–3, 15 May 2009, Pages 220–225
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us