fulltext.study @t Gmail

Biocatalytic esterification of citronellol with lauric acid by immobilized lipase on aminopropyl-grafted mesoporous SBA-15

Paper ID Volume ID Publish Year Pages File Format Full-Text
4278 218 2009 8 PDF Available
Title
Biocatalytic esterification of citronellol with lauric acid by immobilized lipase on aminopropyl-grafted mesoporous SBA-15
Abstract

Mesoporous SBA-15 was synthesized under acidic condition at 40 °C with a non-ionic triblock copolymer (P123) as the template. The synthesis gel composition used was 1 SiO2:0.017 P123:2.9 HCl:202.6 H2O. Functionalization of SBA-15 with 3-aminopropyltriethoxysilane (APTES) by post-synthesis method was performed under reflux for 2 h. The mesoporous samples were characterized using Fourier transform infrared (FT-IR), nitrogen adsorption, transmission electron microscopy (TEM) and scanning electron microscopy (SEM). They were then utilized as supports for the immobilization of lipase to be subsequently used for the esterification of citronellol and lauric acid. Leaching and reusability tests were also conducted on the immobilized enzymes. Functionalization resulted in about 10% improvement in enzyme loading, leading to higher activity. The immobilized enzyme was also more stable to low pH and high temperature while showing better retention (up to 95%) of enzyme molecules. Immobilized lipase maintained 90% of its esterification activity in non-aqueous system even after 4 cycles of use. The improvements were associated with enhanced surface hydrophobicity, changes in pore shapes and stronger enzyme–support interactions with minimal effects to the enzymatic activity.

Keywords
Biocatalyst preparation; Solid-state; Functionalized SBA-15; Enzyme; Immobilized; Citronellyl laurate
First Page Preview
Biocatalytic esterification of citronellol with lauric acid by immobilized lipase on aminopropyl-grafted mesoporous SBA-15
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 44, Issues 2–3, 15 May 2009, Pages 263–270
Authors
, , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us