fulltext.study @t Gmail

Matrix-driven formation of mesenchymal stem cell–extracellular matrix microtissues on soft alginate hydrogels

Paper ID Volume ID Publish Year Pages File Format Full-Text
429 42 2014 12 PDF Available
Title
Matrix-driven formation of mesenchymal stem cell–extracellular matrix microtissues on soft alginate hydrogels
Abstract

Mesenchymal stem cells (MSCs) can be made to rearrange into microtissues in response to specific matrix cues, a process that depends on a balance between cell–matrix and cell–cell interactions. The effect of such cues, and especially their interplay, is still not fully understood, particularly in three-dimensional (3-D) systems. Here, the behaviour of human MSCs cultured within hydrogel matrices with tailored stiffness and composition was evaluated. MSC aggregation occurred only in more compliant matrices (G′ ⩽ 120 Pa), when compared to stiffer ones, both in the presence and in the absence of matrix-bound arginine–glycine–aspartic acid cell–adhesion ligands (RGD; 0, 100 and 200 μM). Fibronectin assembly stabilized cell–cell contacts within aggregates, even in non-adhesive matrices. However, MSCs were able to substantially contract the artificial matrix only when RGD was present. Moreover, compliant matrices facilitated cell proliferation and provided an environment conducive for MSC osteogenic differentiation, even without RGD. Cell interactions with the original matrix became less important as time progressed, while the de novo-produced extracellular matrix became a more critical determinant of cell fate. These data provide further insights into the mechanisms by which MSCs sense their microenvironment to organize into tissues, and provide new clues to the design of cell-instructive 3-D matrices.

Graphical abstractFigure optionsDownload full-size imageDownload high-quality image (124 K)Download as PowerPoint slide

Keywords
Hydrogels; Viscoelastic properties; Cell traction; Cell–matrix interactions; Cell aggregation
First Page Preview
Matrix-driven formation of mesenchymal stem cell–extracellular matrix microtissues on soft alginate hydrogels
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 10, Issue 7, July 2014, Pages 3197–3208
Authors
, , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us