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Development of a fed-batch process for the production of anticancer drug TATm-survivin(T34A) in Escherichia coli

Paper ID Volume ID Publish Year Pages File Format Full-Text
4294 219 2009 6 PDF Available
Title
Development of a fed-batch process for the production of anticancer drug TATm-survivin(T34A) in Escherichia coli
Abstract

A fed-batch process was developed for intracellular production of recombinant TATm-survivin(T34A) in Escherichia coli under the control of T7 promoter. The effects of induction mode and nutritional conditions were investigated. Compared to the one-point addition of inducer, the step-wise addition of isopropyl beta-d-thiogalactopyranoside (IPTG) maintained higher plasmid stability and increased the production level by 52%. Insufficient glucose supply after induction was observed to control acetate accumulation effectively and improved the expression of the target gene evidently. Remarkably, the pre-induction supplement of inorganic nitrogen source had a positive influence on the production of TATm-survivin(T34A). High ammonium concentration of 4.8 g l−1 was the most efficient in enhancing the production level (as the percentage of total cellular protein) and the specific productivity of TATm-survivin(T34A). As a result, the production of TATm-survivin(T34A) was optimized from 11.6% to 36.8% of total cellular protein (corresponding to 1.68 g l(1). The findings provide valuable information for optimization of recombinant protein expression.

Keywords
TATm-survivin(T34A); Escherichia coli; Fed-batch culture; Optimization; Acetic acid; Bioprocess design
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Development of a fed-batch process for the production of anticancer drug TATm-survivin(T34A) in Escherichia coli
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 43, Issue 2, 15 February 2009, Pages 163–168
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us