fulltext.study @t Gmail

Surface energetics to assess biomass attachment onto hydrophobic interaction adsorbents in expanded beds

Paper ID Volume ID Publish Year Pages File Format Full-Text
4310 220 2009 11 PDF Available
Title
Surface energetics to assess biomass attachment onto hydrophobic interaction adsorbents in expanded beds
Abstract

Cell-to-support interaction and cell-to-cell aggregation phenomena have been studied in a model system composed of intact yeast cells and Phenyl-Streamline adsorbents. Biomass components and beaded adsorbents were characterized by contact angle determinations with three diagnostic liquids and zeta potential measurements. Subsequently, free energy of interaction vs. distance profiles between interacting surfaces was calculated in the aqueous media provided by operating mobile phases. The effect of pH and ammonium sulphate concentration within the normal operating ranges was evaluated. Calculation indicated that moderate interaction between cell particles and adsorbent beads can develop in the presence of salt. Cell-to-cell aggregation was suspected to occur at high salt concentration and neutral pH. Predictions based on the application of the XDLVO approach were confirmed by independent experimental methods like biomass deposition experiments and laser diffraction spectroscopy. Understanding biomass attachment onto hydrophobic supports can help in alleviating process limitations normally encountered during expanded bed adsorption of bioproducts.

Keywords
Direct sequestration; Biomass attachment; Fluidized beds; Sorption performance; Surface energetics; Bioprocess design; Hydrophobic interaction
First Page Preview
Surface energetics to assess biomass attachment onto hydrophobic interaction adsorbents in expanded beds
Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 43, Issue 1, 15 January 2009, Pages 16–26
Authors
, , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering