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Influence of temperature on protein hydrolysis in a cyclic batch enzyme membrane reactor

Paper ID Volume ID Publish Year Pages File Format Full-Text
4328 221 2008 7 PDF Available
Title
Influence of temperature on protein hydrolysis in a cyclic batch enzyme membrane reactor
Abstract

A cyclic batch enzyme membrane reactor (CBEMR) incorporating a 8000-Da polyethersulphone membrane was intended for enhancing the enzyme (Protex 6L from Bacillus licheniformis, EC. 3.4.21.62) use in the production of a whey protein hydrolysate. A mechanistic mathematical model comprising zero-order kinetics for the hydrolysis and second-order deactivation for the enzyme was proposed and validated through experiments. The influence of reaction temperature was studied and process optimisation (given the production requirements) was performed in terms of number of batch reactions that minimise the total amount of enzyme used. The optimal operation of the CBEMR allowed savings of up to 44 and 32% of enzyme compared to the single batch operation mode at 50 and 60 °C, respectively. No enzyme savings were detected when temperature was fixed at 70 °C. In general, the optimal operation temperature was 60 °C, yielding lower enzyme consumption for all productivities of the reactor.

Keywords
Membrane bioreactors; Proteolysis; Enzyme bioreactors; Optimisation; Whey protein hydrolysates; Enzyme reuse
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 42, Issue 3, 1 December 2008, Pages 217–223
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
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Any Questions? feel free to contact us