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Effect of cra gene knockout together with other genes knockouts on the improvement of substrate consumption rate in Escherichia coli under microaerobic condition

Paper ID Volume ID Publish Year Pages File Format Full-Text
4329 221 2008 5 PDF Available
Title
Effect of cra gene knockout together with other genes knockouts on the improvement of substrate consumption rate in Escherichia coli under microaerobic condition
Abstract

The catabolite repressor/activator protein Cra (FruR) is known to repress the glycolysis pathway genes and activate the gluconeogenic pathway genes. Disruption of cra gene may, therefore, activate glycolysis and thus improve the glucose consumption rate, which may in turn lead to the increase of the specific metabolite production rate. However, it was found in our previous investigation that the substrate consumption rate could not be improved for the aerobic cultivation of cra mutant due to activation of Entner–Doudoroff (ED) pathway, and the repression of the TCA cycle caused by the down-regulation of icdA and aceBAK genes. To overcome this problem, we investigated the effects of edd and arcA genes knockout on the glucose consumption rate. The cra.edd mutant showed the higher glucose uptake rate as compared with the wild type under microaerobic condition. Since arcA gene knockout mutant repressed pfl operon under microaerobic condition, the glucose consumption rate was not improved for cra.edd.arcA mutant. In order to show that the increase in the glucose consumption rate can lead to the increase of the metabolite production rate, we considered the lactate production by pflA mutant. It was shown that the substrate consumption rate could be significantly improved for cra.pflA mutant as compared with pflA mutant, and thus the lactate production rate could be improved by this double mutant if fructose was used as a carbon source.

Keywords
Escherichia coli; Cra mutant; Cra.pflA mutant; Cra.edd double mutant; Cra.edd.arcA mutant; Glucose consumption rate; Lactate fermentation
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Effect of cra gene knockout together with other genes knockouts on the improvement of substrate consumption rate in Escherichia coli under microaerobic condition
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 42, Issue 3, 1 December 2008, Pages 224–228
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us