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Study on factors influencing stability of whole cell during biodiesel production in solvent-free and tert-butanol system

Paper ID Volume ID Publish Year Pages File Format Full-Text
4363 223 2008 5 PDF Available
Title
Study on factors influencing stability of whole cell during biodiesel production in solvent-free and tert-butanol system
Abstract

Utilizing whole cell biocatalyst for biodiesel production has some advantages since it can avoid the complex procedures of isolation, purification and immobilization of extracellular lipase. However, during repeated use of Rhizopus oryzae (R. oryzae) IFO4697 whole cell for biodiesel production in solvent-free system, the whole cell exhibited very poor stability; while the whole cell stability has been found to be significantly improved in tert-butanol system compared to that in solvent-free system. The difference in whole cell stability was found to be due to the difference of product accumulation between solvent-free and tert-butanol system. After 144 h reaction, glycerol and methyl ester accumulated in the cell in solvent-free system came up to about 1000 mg/g and 350 mg/g dry biomass, respectively, while in tert-butanol system, glycerol and methyl ester accumulation was kept at a relatively low level, approximately 100 mg/g and 2 mg/g dry biomass, respectively. The accumulated glycerol influenced whole cell stability through mass transfer limitation only, while the accumulated methyl ester influenced whole cell stability through both mass transfer limitation and product inhibition. Further study showed that a slight loss in enzymatic activity in tert-butanol system was caused by protein leakage.

Keywords
Biodiesel; Immobilized cells; Stability; Bioconversion; Enzyme biocatalysis; Product inhibition
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 41, Issue 2, 1 September 2008, Pages 111–115
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us