fulltext.study @t Gmail

Separation of recombinant human vasoactive intestinal peptide–humanin fusion protein via three different approaches

Paper ID Volume ID Publish Year Pages File Format Full-Text
4364 223 2008 6 PDF Available
Title
Separation of recombinant human vasoactive intestinal peptide–humanin fusion protein via three different approaches
Abstract

Both vasoactive intestinal peptide (VIP) and humanin (HN) can provide neuroprotection against β-amyloid toxicity and are believed to be beneficial in the treatment of Alzheimer's disease. For the sake of tandem co-expression of recombinant human VIP and HN(rhVIP-HN) fusion protein, new VIP-HN gene is constructed and cloned into the plasmid pET28a(+) and is expressed in Escherichia coli BL21(DE3). Since the fusion protein was present as inclusion bodies, three different preparation approaches are employed to obtain the hexa-histidine tagged rhVIP-HN (His6-rhVIP-HN) fusion protein by Ni2+ chelating resin chromatography. The comparison of the results obtained from the three approaches reveals that renaturation during the separation process (Approach III) is the most efficient for large-scale preparation of His6-rhVIP-HN. Ultimately, 46 mg of the target protein was obtained from one gram of the inclusion body with purity up to 90%. Since there is not separated step in renaturation procedure, Approach III could be more time-saving, buffer-saving and easy to operate than the other two approaches. These results can be useful for preparation of rhVIP-HN in large scale.

Keywords
Bioseparations; Protein denaturation; Refolding; Protein recovery; Recombinant DNA; Vasoactive intestinal peptide
First Page Preview
Separation of recombinant human vasoactive intestinal peptide–humanin fusion protein via three different approaches
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 41, Issue 2, 1 September 2008, Pages 116–121
Authors
, ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us