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Stability and catalytic properties of encapsulated subtilisin in xerogels of alkoxisilanes

Paper ID Volume ID Publish Year Pages File Format Full-Text
43773 45987 2008 6 PDF Available
Title
Stability and catalytic properties of encapsulated subtilisin in xerogels of alkoxisilanes
Abstract

Subtilisin was encapsulated in glass sol–gel matrices using alkoxysilane precursors of different chain lengths. The entrapment efficiency of the sol–gel glass was about 80%. The resultant glass enzyme had the same optimum pH of 7.0, but the optimum temperature was shifted to a higher temperature of 60 °C. The biocatalyst sol–gel particles retained 50% of the original activity even after 11 cycles of repeat use. The scanning electron micrograph of the immobilized enzyme showed uniform round particles of 5–20 μm. The specific surface area by BET measurement of the immobilized subtilisin in vinyl tri methoxy silane (VTMS) was found to be 38 m2 g−1. This immobilized enzyme was useful for the synthesis of peptides either in a mixture of acetonitrile: dimethyl formamide (DMF) or in 1-butyl 3-methyl imidazolium hexaflurophosphate, an ionic liquid. The formation of dipeptides and tripeptides of l-alanine was confirmed by TLC, HPLC and FT-IR analysis.

Graphical abstractSubtilisin was encapsulated in glass sol–gel matrices using alkoxysilane precursors of different chain lengths. The activity retention of the sol–gel glass was about 80%. The sol–gel subtilisin having high catalytic efficiency with high thermal stability and was used for single step peptide synthesis.Figure optionsDownload full-size imageDownload as PowerPoint slide

Keywords
VTMS, vinyl tri methoxy silane; MTMS, methyl tri methoxy silane; TEOS, tetra ethoxy silane; DMF, dimethyl formamide; IL, ionic liquid; BIMMPF6, 1-butyl-3-methylimidazolium hexafluorophosphate; TLC, thin layer chromatography; HPLC, high performance liquid
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Stability and catalytic properties of encapsulated subtilisin in xerogels of alkoxisilanes
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Publisher
Database: Elsevier - ScienceDirect
Journal: Applied Catalysis A: General - Volume 341, Issues 1–2, 15 June 2008, Pages 168–173
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Catalysis
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Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
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Any Questions? feel free to contact us