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High-pressure gas-assisted absorption of protein within biopolymeric micro-patterned membrane

Paper ID Volume ID Publish Year Pages File Format Full-Text
4386 224 2008 8 PDF Available
Title
High-pressure gas-assisted absorption of protein within biopolymeric micro-patterned membrane
Abstract

The loading of bio-molecules within polymeric matrices, such as particles, sponges or thin membranes, by completely solvent-free processes has an enormous interest in biomedical engineering applications, in particular, for the production of drug carriers and biomimetic scaffolds. In this research, we developed a process based on the use of high-pressure carbon dioxide (CO2) in order to absorb bioactive molecules within poly(lactic-co-glycolic acid) (PLGA). A dye, methylene blue, was used to optimize process conditions, whereas lysozyme was used to evaluate the protein-loading efficiency. The in situ UV–vis measurements of the gas-assisted absorption of methylene blue showed that the dye concentration within the polymer increased tenfold with respect to the control sample, after 35 min at a relatively low pressure of 2.0 MPa. Experimental measurements in the range of 0.1–6.7 MPa, and at 308 and 313 K, showed that the protein loading was highly enhanced by CO2 assisted absorption, whereas no detectable amount of protein was observed in the control. This gas-assisted technique is a completely solvent-free process, operates at mild temperature conditions (<313 K), and is particularly promising for the absorption of thermo-degradable molecules within PLGA membranes without affecting their morphology at macro- and micro-scale.

Keywords
Absorption; Protein; PLGA; High pressure; Biomimetic scaffold; In situ UV–vis measurement; Diffusion coefficient
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High-pressure gas-assisted absorption of protein within biopolymeric micro-patterned membrane
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 40, Issue 2, 1 June 2008, Pages 241–248
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us