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Enzymatic synthesis of conjugated linoleoyl ascorbate in acetone

Paper ID Volume ID Publish Year Pages File Format Full-Text
4401 224 2008 5 PDF Available
Title
Enzymatic synthesis of conjugated linoleoyl ascorbate in acetone
Abstract

Conjugated linoleoyl ascorbates were synthesized through the condensation of cis-9,trans-11-conjugated linoleic acid (c9,t11CLA) or trans-10,cis-12-conjugated linoleic acid (t10,c12CLA) with l-ascorbic acid (AsA) using immobilized lipase in a water-soluble organic solvent at 50 °C. The conversion was higher at higher polarity of the solvent and a higher ratio of CLA to AsA. The conversion for the synthesis of t10,c12CLA-AsA was higher than those for c9,t11CLA-AsA and linoleoyl ascorbate (LA-AsA), when Chirazyme® L-2 C2 from Candida antarctica type B, which has been commonly used for enzymatic reaction in organic solvent, was used as a catalyst. The unmodified CLA isomers were almost completely oxidized within 4 h at 65 °C and 75% relative humidity, whereas all the CLA-AsAs were significantly resistant to oxidation. The oxidation kinetics was empirically expressed by the Weibull equation, and the rate constant, k, was estimated. The k values for CLA-AsAs and LA-AsA were about 1/30 and 1/60, respectively, of that for the corresponding unmodified CLA isomers. The enzymatic modification of CLA with AsA was an effective technique for improvement in the oxidative stability of CLA.

Keywords
Conjugated linoleic acid; Immobilized lipase; Linoleoyl ascorbate; Oxidative stability
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Enzymatic synthesis of conjugated linoleoyl ascorbate in acetone
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 40, Issue 2, 1 June 2008, Pages 368–372
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
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Price was $35.95
You save - $31
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