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Mathematical model for change in diameter distribution of baculovirus-infected Sf-9 insect cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
4403 224 2008 8 PDF Available
Title
Mathematical model for change in diameter distribution of baculovirus-infected Sf-9 insect cells
Abstract

Sf-9 insect cells were examined for diameter before and after baculovirus infection. The diameter distribution of uninfected Sf-9 insect cells was described by the normal distribution function with a mean cell diameter and standard deviation of 18.5 ± 1.5 μm. After synchronous infection at a high MOI and high cell density, the infected cells grew in diameter almost linearly with time for about 48 h to have a steady mean diameter 1.45 times larger than that of uninfected cells, though still normally distributed. The distribution of the infected cells began to broaden at around 6 h-post-infection and eventually had standard deviation twice as large as that of uninfected cells. A mathematical model was developed to describe the change in the diameter distribution of virus-infected insect cells, integrating the distributions of infected cell subsets that were generated according to the Poisson distribution function between time t and t + dt and individually characterized with a post-infection time. The growth of uninfected cells and post-infection events such as progeny virus replication and secondary infection of uninfected remained cells were simulated according to a previous report. The model calculation predicts the change in diameter distribution of Sf-9 insect cells that were infected under various conditions, giving an indication to assess the degree of virus infection.

Keywords
Baculovirus; Insect cell; Sf-9; Modelling; Diameter distribution
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Mathematical model for change in diameter distribution of baculovirus-infected Sf-9 insect cells
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 40, Issue 2, 1 June 2008, Pages 379–386
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us