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Monolayer culture of primary rat hepatocytes on an Arg-Gly-Asp (RGD)-immobilized polystyrene dish express liver-specific functions of albumin production and p-acetamidophenol metabolism the same as for spheroid culture

Paper ID Volume ID Publish Year Pages File Format Full-Text
4404 224 2008 5 PDF Available
Title
Monolayer culture of primary rat hepatocytes on an Arg-Gly-Asp (RGD)-immobilized polystyrene dish express liver-specific functions of albumin production and p-acetamidophenol metabolism the same as for spheroid culture
Abstract

The aim of this study was to evaluate the expression of albumin production and drug metabolism activities of primary rat hepatocytes under a two-dimensional monolayer culture condition formed on a RGD-immobilized culture dish. Albumin production activity on the RGD-immobilized dish (2500 ng cm−2 Pronectin F-coated dish) was 1.6 (p < 0.05) and 1.2 (p < 0.05) times higher than that on the collagen-coated dish and that of the spheroid culture, respectively. p-Acetamidophenol (APAP) metabolism rate of the collagen-monolayer at 5 days of culture was decreased to 16% of the activity at 1 day of culture. On the other hand, APAP metabolism activities of the RGD-monolayer and spheroid cultures were well maintained during 7 days of culture. However, we could not detect the effectiveness of the RGD-monolayer on lidocaine metabolism of hepatocytes. This is the first report suggesting that a hepatocyte monolayer on a RGD-immobilized culture substratum expresses albumin production and APAP metabolism activities equal to those of a hepatocyte spheroid culture.

Keywords
Arg-Gly-Asp (RGD); Hepatocyte; Amino acid; Animal cell culture; Tissue cell culture; Bioconversion
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Monolayer culture of primary rat hepatocytes on an Arg-Gly-Asp (RGD)-immobilized polystyrene dish express liver-specific functions of albumin production and p-acetamidophenol metabolism the same as for spheroid culture
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 40, Issue 2, 1 June 2008, Pages 387–391
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us