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Production of ricinoleic acid estolide with free and immobilized lipase from Candida rugosa

Paper ID Volume ID Publish Year Pages File Format Full-Text
4430 226 2008 7 PDF Available
Title
Production of ricinoleic acid estolide with free and immobilized lipase from Candida rugosa
Abstract

Ricinoleic acid estolide was produced by using free and immobilized Candida rugosa lipase at moderate temperature in a bioreactor. This work describes the immobilization of C. rugosa lipase on 10 different supports by covalent binding and physical adsorption, and how of the most suitable immobilized derivative was selected. The comparison was mainly based on the enzyme content and on the activity results. An anion exchange resin was judged to be the most appropriate support and the corresponding immobilization process was investigated and optimized. Although repeated batch reactions using the same derivative are not entirely advisable, the reaction proceeds at a noticeably slower rate and the degree of condensation reached is lower when the same amount of protein as in the derivative is added to the bioreactor in native form.

Keywords
Estolide; Ricinoleic acid; Lipase; Immobilized enzymes; Heterogeneous biocatalysis
First Page Preview
Production of ricinoleic acid estolide with free and immobilized lipase from Candida rugosa
Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 39, Issue 3, 1 May 2008, Pages 450–456
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering