fulltext.study @t Gmail

Kinetic model for DBT desulphurization by resting whole cells of Pseudomonas putida CECT5279

Paper ID Volume ID Publish Year Pages File Format Full-Text
4434 226 2008 10 PDF Available
Title
Kinetic model for DBT desulphurization by resting whole cells of Pseudomonas putida CECT5279
Abstract

Bio-desulphurization kinetics of dibenzothiophene (DBT) using Pseudomonas putida CECT 5279, a genetically modified micro-organism (GMO), is studied. A kinetic model describing the 4S route of DBT desulphurization is proposed. Bio-desulphurization experiments have been carried out using resting whole cells of P. putida CECT 5279 obtained at different growth times as biocatalysts. The kinetic equations proposed for each reaction have been previously checked by studying each reaction of the 4S route individually, employing different substrates in different experiments. Finally, simple Michaelis–Menten kinetic equations for the three first reactions catalyzed by two mono-oxygenases (DszC and DszA) and a kinetic equation taking into account competitive inhibition due to product for the final reaction catalyzed by a desulfinase (DszB) have been adopted. DBT has been desulphurized using cells obtained at different growth times (5, 10, 23, 30 and 45 h). The overall kinetic model proposed involving the four reactions of the 4S route was fitted to all the experimental data yielding a set of kinetic parameters able to describe the system evolution. Cell age has influence on the rates of all the reactions: reactions (1), (2) and (3) present maximum rates for cell grown during 30 h, while reaction (4) shows a maximum rate for cells with around 10 h of growth time. However, affinities of each substrate and the inhibition constant of the last reaction are not influenced by the time of growth.

Keywords
Kinetic modelling; Dibenzothiophene; 4S route; Pseudomonas putida; Biodesulphurization; Kinetic parameters; Growth; Product inhibition
First Page Preview
Kinetic model for DBT desulphurization by resting whole cells of Pseudomonas putida CECT5279
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 39, Issue 3, 1 May 2008, Pages 486–495
Authors
, , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us