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Assessment of the stability of TGFβ3 bioactivity for potential bioreactor applications

Paper ID Volume ID Publish Year Pages File Format Full-Text
4447 226 2008 4 PDF Available
Title
Assessment of the stability of TGFβ3 bioactivity for potential bioreactor applications
Abstract

In order to develop suitable bioreactor systems and processes for automated and standardized cell cultures involving the use of bioactive factors, we determined the stability of transforming growth factor beta 3 (TGFβ3) over storage time and under conditions typically used for mammalian cell culture. Using a reporter gene assay with firefly luciferase as readout, significant reduction of TGFβ3 bioactivity was detected to occur both in serum containing medium (SCM) and serum free medium (SFM). The residual activity, quantified by parallel line assays, progressively decreased with time, down to 60% in SCM and 84% in SFM after 1 week at 37 °C, with no further decrease until 3 weeks, whereas such loss could not be predicted using a conventional ELISA method. The reduction of TGFβ3 bioactivity had a negligible influence in a typical biological assay (e.g., chondrocyte proliferation), supporting the possibility of prolonged storage of medium pre-supplemented with TGFβ3 for bioreactor-based chondrocyte expansion. With the ultimate goal of defining suitable operating protocols for automated cell culture bioreactors, the proposed approach should be extended to assessing the stability of other possibly labile medium supplements.

Keywords
Growth factor; Tissue engineering; Bioassay; Automation; Standardization; Bioprocess
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 39, Issue 3, 1 May 2008, Pages 586–589
Authors
, , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us