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Towards information-rich bioprocessing: Generation of spatio-temporal profiles through the use of design of experiments to determine optimal number and location of sensors—An example in thermal profiles

Paper ID Volume ID Publish Year Pages File Format Full-Text
4454 227 2008 7 PDF Available
Title
Towards information-rich bioprocessing: Generation of spatio-temporal profiles through the use of design of experiments to determine optimal number and location of sensors—An example in thermal profiles
Abstract

Stem cell and tissue bioprocessing for regenerative medicine require robust, reproducible, controllable, and optimal processes. In vivo, the body utilises real-time, on-line, in situ monitoring to deliver this level of control. Increasingly, monitoring is becoming more applicable and extremely important in tissue engineering and regenerative medicine due to the advances in monitoring techniques, including the use of biosensors. However, determination of optimal sensor location and number remains to be explored. Herein, we have presented the novel application of design of experiments methodology to address this issue. A culture chamber (equivalent to a 6-well plate) was fabricated and temperature sensors were employed in studying the temperature profile of the chamber under both static and perfused (0.01 mL/min) conditions. Heating elements were used to provide a constant heat source in order to simulate the production of metabolites by the cells. The design of experiments determined the use of nine sensors at both factorial and axial locations. In comparison, two random designs utilising five and seven sensors confirmed that the design of experiments-generated data were information-rich and produced the spatial (and temporal) temperature profiles for the chamber. The implementation of this powerful technique would offer knowledge of the local concentration gradients of important culture parameters allowing for the real-time, local, ‘intelligent’ bioprocess control.

Keywords
Stem cells; Tissue engineering; Bioprocessing; Monitoring; Sensors; Design of experiments
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Towards information-rich bioprocessing: Generation of spatio-temporal profiles through the use of design of experiments to determine optimal number and location of sensors—An example in thermal profiles
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 40, Issue 1, 15 May 2008, Pages 1–7
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us