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Joint effect of nitrogen and phosphorous on glucose oxidase production by Aspergillus niger: Discussion of an experimental design with a risk of co-linearity

Paper ID Volume ID Publish Year Pages File Format Full-Text
4460 227 2008 10 PDF Available
Title
Joint effect of nitrogen and phosphorous on glucose oxidase production by Aspergillus niger: Discussion of an experimental design with a risk of co-linearity
Abstract

The combined effects of nitrogen and phosphorous on the production of glucose oxidase and gluconic acid by Aspergillus niger cannot be adequately described with Monod-type model, neither do they fit well to linear equations with interactions N × P, nor quadratic with N2 and P2 terms. On the other hand, the interactions of type N2P and NP2, although common in real cases such as enzymatic kinetics in the presence of inhibitors, should be verified – if included in empiric models – by means of designs that can lead to artefactual results derived from the co-linearity. To avoid this risk we propose a procedure, based on the ‘bootstrap’ algorithm, which provided consistent results in the mentioned bioproductions. Applied together with methods of response surface and gradient, said procedure allowed to optimize the enzyme production as a function of the concentrations of N and P, to quintuple the initially obtained levels, and to explain other culture behaviours related with the sources of these nutrients.

Keywords
GOD production; Gluconic acid production; Bootstrap algorithm; Factorial design
First Page Preview
Joint effect of nitrogen and phosphorous on glucose oxidase production by Aspergillus niger: Discussion of an experimental design with a risk of co-linearity
Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 40, Issue 1, 15 May 2008, Pages 54–63
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering