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Effect of gamma-ray on activity and stability of alcohol-dehydrogenase from Saccharomyces cerevisiae

Paper ID Volume ID Publish Year Pages File Format Full-Text
4474 227 2008 5 PDF Available
Title
Effect of gamma-ray on activity and stability of alcohol-dehydrogenase from Saccharomyces cerevisiae
Abstract

The effect of γ-ray irradiation on alcohol-dehydrogenase activity of yeast was investigated. The results suggested that low doses of γ-ray (10 and 20 Gy) significantly increased the enzyme activity. This work also describes the impact of irradiation on immobilization efficiency of biocatalyst entrapped on to alginate gel beads. When yeast irradiated to a dose of 20 Gy was immobilized, ADH stability was improved up to 1.4 times at 45 °C compared to the immobilized non-irradiated cells. Also, the irradiated biocatalyst, when immobilized, can be reused more than eight times in oxidation reaction of ethanol. This preparation also permitted to reach high yields of immobilization (79%) and activity (88%).

Keywords
Alcohol-dehydrogenase; Entrapment; Immobilization; γ-ray; Saccharomyces cerevisiae
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Effect of gamma-ray on activity and stability of alcohol-dehydrogenase from Saccharomyces cerevisiae
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 40, Issue 1, 15 May 2008, Pages 184–188
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
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