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Purification of phenylalanine ammonia-lyase in PEG1000/Na2SO4 aqueous two-phase system by a two-step extraction

Paper ID Volume ID Publish Year Pages File Format Full-Text
4478 228 2007 7 PDF Available
Title
Purification of phenylalanine ammonia-lyase in PEG1000/Na2SO4 aqueous two-phase system by a two-step extraction
Abstract

The effects of molecular weight and concentration of PEG and various salts on the partitioning of PAL from Rhodotorula glutinis were studied in PEG/salts aqueous two-phase systems at pH 8.8 and 25 °C. With the application of 11.0%PEG1000/14.0%Na2SO4, and 11.0%PEG1000/14.0%Na2SO4/5.3%Na2CO3, purification of PAL by a two-step extraction was proposed in this work. Compared to the conventional salting-out step, 3.7 times of the purification factor (9.3-fold) and a comparable recovery yield (80.6%) were obtained in this method. Therefore, the work offers a useful method for PAL purification and the two-step extraction could replace the conventional salting-out step in the purification.

Keywords
Aqueous two-phase system; Phenylalanine ammonia-lyase; Partition; PEG; Salt
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Purification of phenylalanine ammonia-lyase in PEG1000/Na2SO4 aqueous two-phase system by a two-step extraction
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 37, Issue 3, 15 December 2007, Pages 231–237
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
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