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Structured parallel diffusion model for intraparticle mass transport of proteins to porous adsorbent

Paper ID Volume ID Publish Year Pages File Format Full-Text
4487 228 2007 13 PDF Available
Title
Structured parallel diffusion model for intraparticle mass transport of proteins to porous adsorbent
Abstract

A structured parallel diffusion model (SParD model) has been developed to describe the intraparticle mass transport of proteins in porous chromatographic matrices. This model takes into account surface diffusion, hindered pore diffusion, pore size distribution of porous supports, and the shrinking of effective pore radius by protein adsorption. Moreover, the Maxwell–Stefan approach was adopted to express the surface diffusion. The uptake kinetics of proteins, bovine serum albumin and γ-globulin, to anion exchanger, DEAE Spherodex M, is studied by batch adsorption. The dynamic adsorption data thus obtained are analyzed by the SParD model. The surface diffusivities of the two proteins are acquired by matching the model with experimental data. The hindrance effect on pore diffusion in different-sized pores was illuminated by the calculated intraparticle concentration profiles of adsorbed protein and the ratios of surface diffusion to pore diffusion rates in different pores. Thus, the validity and usefulness of the SParD model have been confirmed.

Keywords
Mass transport; Structured parallel diffusion model; Protein adsorption; Pore size distribution; Hindered pore diffusion; Surface diffusion
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 37, Issue 3, 15 December 2007, Pages 298–310
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
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Price was $35.95
You save - $31
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