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Phase equilibrium and protein partitioning in aqueous two-phase systems containing ammonium carbamate and block copolymers PEO–PPO–PEO

Paper ID Volume ID Publish Year Pages File Format Full-Text
4488 228 2007 8 PDF Available
Title
Phase equilibrium and protein partitioning in aqueous two-phase systems containing ammonium carbamate and block copolymers PEO–PPO–PEO
Abstract

The liquid–liquid equilibrium of aqueous two-phase systems formed by the PEO–PPO–PEO block copolymers F38 or F68 and ammonium carbamate and the partition of the model proteins bovine serum albmin, lysozyme, α-lactalbumin, β-lactoglobulin and γ-globulin in these systems are presented in this paper. Phase diagrams were determined at 4 °C (F38) and 25 °C (F38 and F68); systems containing F68 present a narrow two-phase region, while systems containing F38 have a suitable working region. Partition coefficients, defined as the ratio of protein concentration in the polymer-rich phase to that in the salt-rich phase, were found to vary in the range of 0.1 to 0.8 for bovine serum albumin, 0.5 to 2.0 for lysozyme, 1.0 to 2.5 for α-lactalbumin, 0.1 to 1.0 for β-lactoglobulin and 0.3 to 1.0 for γ-globulin, depending on the polymer chain-size, temperature and tie-line length. The different trends of protein partition coefficients show that high degrees of separation can be achieved, establishing the system F38 and ammonium carbamate as an alternative to be considered when planning the downstream processing of proteins.

Keywords
Protein; Downstream processing; Aqueous two-phase systems; Salt; Polymer
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Phase equilibrium and protein partitioning in aqueous two-phase systems containing ammonium carbamate and block copolymers PEO–PPO–PEO
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 37, Issue 3, 15 December 2007, Pages 311–318
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us