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Fabrication of superporous cellulose beads with grafted anion-exchange polymer chains for protein chromatography

Paper ID Volume ID Publish Year Pages File Format Full-Text
4491 228 2007 6 PDF Available
Title
Fabrication of superporous cellulose beads with grafted anion-exchange polymer chains for protein chromatography
Abstract

Glycidyl methacrylate was grafted onto the pore surface in superporous cellulose beads using ceric ammonium nitrate as initiator. By modifying the grafted and ungrafted matrices with diethylaminoethyl (DEAE) groups, anion exchangers were produced. Under a proper grafting reaction condition, the adsorption capacity of bovine serum albumin (BSA) to the DEAE-G-SC was over two times increased as compared to the ungrafted DEAE-SC and no nonspecific adsorption of BSA was observed. Moreover, the intraparticle diffusive mass transfer was not affected by this grafted polymer chains. However, the flow hydrodynamic experiments revealed that the grafted polymer chains led to the decrease of flowthrough pores. So the convective mass transport in the particles was significantly reduced, leading to the decrease of column efficiency with flow velocity. Despite this unfavorable effect, however, due to the high adsorption capacity of the grafted beads, the DEAE-G-SC column could still be operated at a DBC of 20–35% higher than the normal DEAE-SC column in the range of 150–600 cm/h.

Keywords
Grafting polymerization; Superporous cellulose bead; Anion exchanger; Protein adsorption; High capacity; Chromatography
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Fabrication of superporous cellulose beads with grafted anion-exchange polymer chains for protein chromatography
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 37, Issue 3, 15 December 2007, Pages 332–337
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us