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Culture-based strategies to enhance cellulase enzyme production from Trichoderma reesei RUT-C30 in bioreactor culture conditions

Paper ID Volume ID Publish Year Pages File Format Full-Text
4508 230 2008 9 PDF Available
Title
Culture-based strategies to enhance cellulase enzyme production from Trichoderma reesei RUT-C30 in bioreactor culture conditions
Abstract

Filamentous fungi Trichoderma reesei are considered to be one of the most efficient hyper producers of cellulase that is used in industry. Cellulase production by T. reesei was carried out using high concentration of cellulose to substitute glucose with the aim to improve cellulase production while trying to reduce production costs. The experiments were conducted separately as fed batch growth with T. reesei cultured using four media in a 7 L stirred tank bioreactor. A mixture of lactose and lactobionic acid was added into the bioreactor as cellulase inducers. The use of a cellulose–yeast extract culture medium yielded the highest enzyme and cell production with a volumetric enzyme activity of 69.8 U L−1 h−1, a filter paper activity of 5.02 U mL−1, a CMCase activity of 4.2 U mL−1, and a fungal biomass of 14.7 g L−1. The biomass concentration as a function of time was constant with relatively rapid, early growth on easily metabolized growth medium components (yeast extract), followed by a second slower growth phase due to hydrolysis of cellulose, which follow cellulase concentration augmentation. The costs to produce 1 L of production medium in laboratory-scale experiments were calculated to compare the tested media.

Keywords
Cellulose; Lactose; Medium composition; Trichoderma reesei; Cellulase enzyme
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Culture-based strategies to enhance cellulase enzyme production from Trichoderma reesei RUT-C30 in bioreactor culture conditions
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 40, Issue 3, 1 July 2008, Pages 399–407
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us