fulltext.study @t Gmail

Refolding of a recombinant organic solvent-stable lipase, which is overexpressed and forms an inclusion body, and activation with lipase-specific foldase

Paper ID Volume ID Publish Year Pages File Format Full-Text
4522 230 2008 5 PDF Available
Title
Refolding of a recombinant organic solvent-stable lipase, which is overexpressed and forms an inclusion body, and activation with lipase-specific foldase
Abstract

Overexpression of the organic solvent-stable LST-03 lipase from organic solvent-tolerant Pseudomonas aeruginosa LST-03 was attempted using a recombinant Escherichia coli system. It was achieved by deleting the signal peptide of the lipase and using T7 promoter. Although, the expressed lipase formed an inclusion body in E. coli, it was completely solubilized with 4 M urea and 0.05 mM dithiothreitol. The denaturants used for solubilization of the lipase inclusion body were easily removed by dilution or dialysis. The solubilized lipase was effectively activated by incubation with a lipase-specific foldase. The purity of the lipase recovered from the inclusion body was high. Use of a recombinant E. coli system improved the productivity of the highly pure lipase by about 807-fold, compared to the original system using P. aeruginosa LST-03.

Keywords
Organic solvent stability; Lipase; Chaperonins; Refolding; Protein denaturation; Pseudomonas aeruginosa
First Page Preview
Refolding of a recombinant organic solvent-stable lipase, which is overexpressed and forms an inclusion body, and activation with lipase-specific foldase
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 40, Issue 3, 1 July 2008, Pages 507–511
Authors
, , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us