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Isolation and purification of bacterial poly(3-hydroxyalkanoates)

Paper ID Volume ID Publish Year Pages File Format Full-Text
4531 231 2008 13 PDF Available
Title
Isolation and purification of bacterial poly(3-hydroxyalkanoates)
Abstract

The isolation and the purification of bacterial polyhydroxyalkanoates are the key step of the process profitability in the fermentation system. That is why many scientists have studied this field for the production of this biodegradable polymer. The ideal method should lead to a high purity and recovery level at a low production cost. This paper reviews four isolation methods, i.e. solvent extraction of halosolvent and nonhalosolvent, digestion of non-polyhydroxyalkanoate cell material involving surfactants, sodium hypochlorite or enzyme, mechanical cell disruption methods like using bead mills and high pressure homogenization, and new methods like spontaneous liberation of poly(3-hydroxybutyrate), dissolved air flotation, air classification, or by using supercritical CO2. The pretreatment of cell disruption and the purification methods and analytical methods of polyhydroxyalkanoates are also presented.

Keywords
Biopolymers; Polyhydroxyalkanoates; Recovery; Isolation; Purification; Bacteria
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 39, Issue 1, 1 April 2008, Pages 15–27
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us