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Response surface optimization of medium composition for alkaline protease production by Bacillus clausii

Paper ID Volume ID Publish Year Pages File Format Full-Text
4533 231 2008 6 PDF Available
Title
Response surface optimization of medium composition for alkaline protease production by Bacillus clausii
Abstract

A culture medium for production of bacterial alkaline protease was developed following preliminary studies by means of response surface method. Central composite design was applied to optimize the medium constituents and explain the combined effects of three medium constituents, viz., sucrose, yeast extract and potassium nitrate. The optimum values for the tested variables were; sucrose 11 g l−1, yeast extract 5 g l−1 and KNO3 5.2 g l−1. A second-order model equation was suggested and then validated experimentally. The model adequacy was very satisfactory as the coefficient of determination was 0.94. The maximum alkaline protease production was 1520 ± 35 U ml−1at 42 h of incubation which showed about 1.5-fold increase in protease production over the central point and an overall 6-fold enhancement (1520–250 U ml−1) over the basal medium.

Keywords
Alkaline protease; Bacillus clausii; Medium optimization; Response surface methodology
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 39, Issue 1, 1 April 2008, Pages 37–42
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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