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A short recursive procedure for evaluating effectiveness factors for immobilized enzymes with reversible Michaelis–Menten kinetics

Paper ID Volume ID Publish Year Pages File Format Full-Text
4536 231 2008 8 PDF Available
Title
A short recursive procedure for evaluating effectiveness factors for immobilized enzymes with reversible Michaelis–Menten kinetics
Abstract

A short recursive procedure for calculating the effectiveness factor for enzymes immobilized in porous spherical particles is presented. The method is mathematically simple and very precise; it is valid for reversible Michaelis–Menten kinetics including, as particular situations, simple Michaelis–Menten and product competitive inhibition kinetics.The procedure is a modification of the two-parameter model previously published by the authors. The definition of an auxiliary dimensionless concentration leads to a recursive equation which simplifies the resolution of the model by means of elementary numerical methods. The solution algorithm has been transformed into a computer program, whose source code is appended.The exact values of the effectiveness factors for zero and first order kinetics, as a function of the Thiele module, were compared with those obtained with the numerical procedure proposed in this work. The good agreement between both results demonstrates the validity of the method.

Keywords
Effectiveness factor; Immobilized enzymes; Diffusion-reaction; Mathematical model
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A short recursive procedure for evaluating effectiveness factors for immobilized enzymes with reversible Michaelis–Menten kinetics
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 39, Issue 1, 1 April 2008, Pages 58–65
Authors
, , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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Full-text PDF Download
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