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Calorimetric on-line monitoring of proteolytic activity of P.aeruginosa cultivated in a bench-scale biocalorimeter

Paper ID Volume ID Publish Year Pages File Format Full-Text
4547 231 2008 8 PDF Available
Title
Calorimetric on-line monitoring of proteolytic activity of P.aeruginosa cultivated in a bench-scale biocalorimeter
Abstract

The aim of this work is to generate thermokinetic data for the production of proteases which would be immensely useful not only for successful scale up of a ferementor for protease production but also to monitor and control of the process by following the heat signals. The biocalorimetric studies prove that the protease production and heat generation profiles are comparable. Oxygen uptake rate was found to correlate well with both biomass growth and heat production rate at all lag, exponential and stationary growth phases. Heat yield due to biomass growth (anabolic) related reactions and substrate uptake (catabolic) related reactions were estimated from the calorimetric experiments and the results were analyzed. Crude protease secreted in calorimeter was extracted separately and characterized by Zymogram and SDS-PAGE analytical techniques. The protease was found to be an alkaline protease. This study reveals that the protease secretion by P. aeruginosa could be monitored effectively in a biological reaction calorimeter. Metabolic heat generated is correlated to protease secretion (non-growth process) for the first time in this study. The heat yields due to biomass growth, substrate uptake and oxygen uptake could help to understand the metabolic process in realistic manner and the results would be useful for bioreactor design and scale up.

Keywords
Alkaline protease; Bio-RC1; P. aeruginosa; Heat generation; SDS-PAGE
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Calorimetric on-line monitoring of proteolytic activity of P.aeruginosa cultivated in a bench-scale biocalorimeter
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 39, Issue 1, 1 April 2008, Pages 149–156
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us