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Design a chemically defined/medically approved medium for cell transplantation according to the metabolic characteristics of microencapsulated cells and the process of encapsulation

Paper ID Volume ID Publish Year Pages File Format Full-Text
4548 231 2008 7 PDF Available
Title
Design a chemically defined/medically approved medium for cell transplantation according to the metabolic characteristics of microencapsulated cells and the process of encapsulation
Abstract

Cell microencapsulation is a promising therapeutic strategy to many diseases; however, the metabolic characteristics of microencapsulated cells and also the optimum microcapsule additives that meet the criteria for long-term functional immunoisolated transplantation have rarely been taken into consideration hitherto. In this work, the optimized amino acid supplements and several chemically defined compounds were applied to cultivation of the microencapsulated CHO cells. The results show that the optimized medium significantly enhances cell survival and, particularly, endostatin secretion of encapsulated CHO cells over a time period of 8 weeks. Furthermore, long-term in vitro stability of alginate–poly-l-lysine–alginate (APA) microcapsules underwent no changes, which indicated that this optimized medium might be feasible to maintain high viability of microencapsulated cell for short periods of time before implantation.

Keywords
Cell transplantation; Microencapsulation; Recombinant CHO cell; Chemically defined medium; Amino acid
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Design a chemically defined/medically approved medium for cell transplantation according to the metabolic characteristics of microencapsulated cells and the process of encapsulation
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 39, Issue 1, 1 April 2008, Pages 157–163
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us