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Response surface modeling of vanillin production by Escherichia coli JM109pBB1

Paper ID Volume ID Publish Year Pages File Format Full-Text
4589 233 2007 8 PDF Available
Title
Response surface modeling of vanillin production by Escherichia coli JM109pBB1
Abstract

The increasing concern for healthy and nutritional products together with the high price of natural vanillin extracted from vanilla pods has stimulated the search for alternative means of natural vanillin production. The present work deals with a ferulic acid to vanillin bioconversion process using the recombinant strain Escherichia coli JM109pBB1. A set of batch bioconversion tests was carried out on aqueous ferulic acid solutions according to a 32 full factorial design selecting the starting biomass concentration (X0) and the initial ferulic acid concentration (S0) as independent variables. The experimental data were analyzed by the response surface methodology (RSM) using a quadratic model for predicting the optimal point. The best starting biomass concentration and initial ferulic acid concentration were found to be 380 mg L−1 and 2.95 mM, respectively. Under these conditions the model predicted a maximum vanillin concentration (Pv)(Pv) of 1.45 mM, a vanillin yield on consumed ferulic acid (Yv)(Yv) of 0.57 mM mM−1, a vanillyl alcohol on consumed ferulic acid (Yal) of 0.39 mM mM−1, a volumetric vanillin productivity (Qv)(Qv) of 0.124 mM h−1 and a specific vanillin productivity (qv)(qv) of 0.047 g g−1 h−1.

Keywords
Vanillin; Ferulic acid; Escherichia coli; Optimization; Experimental design; Response surface methodology
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Response surface modeling of vanillin production by Escherichia coli JM109pBB1
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 36, Issue 3, 1 October 2007, Pages 268–275
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us