fulltext.study @t Gmail

Enhanced biodegradation of caffeine by Pseudomonas sp. using response surface methodology

Paper ID Volume ID Publish Year Pages File Format Full-Text
4592 233 2007 6 PDF Available
Title
Enhanced biodegradation of caffeine by Pseudomonas sp. using response surface methodology
Abstract

Pseudomonas sp. NCIM 5235 capable of degrading high concentrations of caffeine (>5 g/l) has been previously isolated from the soil of coffee plantation area. The critical medium components viz., KH2PO4, Na2HPO4, caffeine and Fe2+ affecting the rate of caffeine degradation were determined by Plackett–Burman design. These critical parameters were further optimized using response surface methodology. The optimum concentrations of KH2PO4, Na2HPO4, caffeine and Fe2+ were found to be 3.4 g/l, 0.352 g/l, 6.4 g/l and 0.075% (w/v), respectively. Under optimal conditions the caffeine degradation rate was increased to 0.18 g/(l h) from 0.1 g/(l h). Although the approach used for optimization is conventional, the increase in the rate of degradation of caffeine is significant. This is the first report on a strain capable of growing at higher concentrations of caffeine (>5 g/l) at maximum rate of degradation (0.18 g/(l h)).

Keywords
Caffeine degradation; Pseudomonas; Medium optimization; Plackett–Burman design; Central composite design
First Page Preview
Enhanced biodegradation of caffeine by Pseudomonas sp. using response surface methodology
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 36, Issue 3, 1 October 2007, Pages 288–293
Authors
, ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us