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Kinetics of soluble and immobilized horseradish peroxidase-mediated oxidation of phenolic compounds

Paper ID Volume ID Publish Year Pages File Format Full-Text
4602 234 2007 10 PDF Available
Title
Kinetics of soluble and immobilized horseradish peroxidase-mediated oxidation of phenolic compounds
Abstract

Horseradish peroxidase (HRP) is an intensively used enzyme in biochemical and medical analyses. It is also proposed for wastewater processing. A bioprocess monitoring method based on co-oxidation of phenol-4-sulfonic acid (PSA) and 4-aminoantipyrine (4-AAP) by immobilized HRP was recently described. In this work a mathematical model that describes a set of reactions that occur in phenol/4-AAP/HRP system upon addition of hydrogen peroxide has been used for the evaluation of the PSA/4-AAP/HRP system by estimating and comparing the apparent kinetic parameters for different reaction systems. The parameters thus obtained were compared for three phenolic compounds (phenol, p-chlorophenol and PSA) and for immobilized HRP in the PSA/4-AAP system. The model was able to capture distinctive features of the system, such as increasing initial reaction rates for increasing concentrations of H2O2; a plateau upon reactant exhaustion; and decreasing initial reaction rates at high H2O2 concentrations. The performance of the PSA/4-AAP/HRP system expressed as the initial reaction rate was found to be less than 12% that of the conventional phenol/4-AAP/HRP system. The estimated parameters for the kinetic model provided the basis for the mechanistic explanation for the lower performance observed in the system containing PSA.

Keywords
Horseradish peroxidase; Kinetic parameters; Modelling; 4-Aminoantipyrine; Immobilization; Biosensors
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Kinetics of soluble and immobilized horseradish peroxidase-mediated oxidation of phenolic compounds
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 35, Issue 2, 15 July 2007, Pages 126–135
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us