fulltext.study @t Gmail

Polypeptide linkers suitable for the efficient production of dimeric scFv in Escherichia coli

Paper ID Volume ID Publish Year Pages File Format Full-Text
4606 234 2007 8 PDF Available
Title
Polypeptide linkers suitable for the efficient production of dimeric scFv in Escherichia coli
Abstract

Single chain variable fragment antibodies (scFvs) were selected from a phage library displaying scFvs consisting of the VH and VL domains of an anti-bisphenol A monoclonal antibody and 20 randomized amino acids linkers by biopanning using an antigen bisphenol A. After four rounds of biopanning selections, seven scFvs with different polypeptide linkers were isolated. The scFvs expressed by the Escherichia coli transformants predominantly formed dimeric structures, and were found in soluble fractions at higher concentrations than those of scFvs containing flexible linkers (G4S)1–3, because the transformants expressing these scFvs could grow to higher cell concentration after the induction of scFv production with IPTG. Consequently, five times higher productivities of soluble scFvs were attained compared with scFvs having the flexible linkers. The antigen binding activities of these scFvs were confirmed by affinity chromatography using an antigen-coupled column and ELISA. The selected polypeptide linkers will be useful for the production of soluble scFv dimers in E. coli.

Keywords
ScFv; Polypeptide linker; Phage display; Dimeric scFv
First Page Preview
Polypeptide linkers suitable for the efficient production of dimeric scFv in Escherichia coli
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 35, Issue 2, 15 July 2007, Pages 158–165
Authors
, , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us