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Production of protein hydrolysates in an enzymatic membrane reactor

Paper ID Volume ID Publish Year Pages File Format Full-Text
4620 235 2008 9 PDF Available
Title
Production of protein hydrolysates in an enzymatic membrane reactor
Abstract

The hydrolysis of casein in the presence of proteolytic enzymes: thermolysin and subtilisin, is discussed. For both biocatalysts an appropriate process temperature was chosen and the kind and values of constants in the kinetic equation were determined. Thermolysin was selected for further studies as the more appropriate catalyst because of a strong inhibition of subtilisin.A model of the process was proposed for enzymatic reaction which takes place in a membrane bioreactor with a complete retention of the enzyme and a partial retention of the substrate in the reaction zone. Having selected a proper membrane, the model was successfully verified using the data from the considered casein hydrolysis. It was proved that at a relatively high substrate retention (i.e. ca. 50%), the substrate conversion degree could increase by ca. 50%. Owing to a high cost of biocatalysts such process intensification is significant. It is all the more promising, as separation of the enzyme on a membrane allows us to use the biocatalyst many times without applying any surface immobilisation techniques.

Keywords
Casein hydrolysates; Membrane bioreactor; Membrane separation; Native enzyme immobilisation; Integrated system
First Page Preview
Production of protein hydrolysates in an enzymatic membrane reactor
Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 39, Issue 2, 15 April 2008, Pages 221–229
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering