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Optics-intrinsic double-circle phenomenon in protein adsorption visualized by confocal laser scanning microscopy

Paper ID Volume ID Publish Year Pages File Format Full-Text
4624 235 2008 9 PDF Available
Title
Optics-intrinsic double-circle phenomenon in protein adsorption visualized by confocal laser scanning microscopy
Abstract

Confocal laser scanning microscopy (CLSM) has been introduced in the research of chromatographic adsorption and the technique provides a direct and noninvasive way to measure and characterize intraparticle concentration distributions of proteins on a single particle level. In this work, an optics-intrinsic double-circle phenomenon that occurs in protein adsorption visualized by CLSM is exhibited and its optical mechanism is reasonably explained by an evolutive version of the attenuation equation. The time course of intraparticle fluorescence intensity profiles obtained from CLSM (even the double-circle phenomenon) in this research is vividly reproduced by model simulation, which takes into account the light attenuation by combining the attenuation equation with an effective pore diffusion model.

Keywords
Protein adsorption; Confocal laser scanning microscopy; Attenuation equation; Pore diffusion model
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Optics-intrinsic double-circle phenomenon in protein adsorption visualized by confocal laser scanning microscopy
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 39, Issue 2, 15 April 2008, Pages 258–266
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us