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Mathematical tools for objective comparison of microbial cultures: Application to evaluation of 15 peptones for lactic acid bacteria productions

Paper ID Volume ID Publish Year Pages File Format Full-Text
4626 235 2008 12 PDF Available
Title
Mathematical tools for objective comparison of microbial cultures: Application to evaluation of 15 peptones for lactic acid bacteria productions
Abstract

Using the logistic model as a starting point, a set of reparameterised equations were established which permit the easy calculation of the relevant parameters of microbial kinetics, together with their confidence limits, with the aim of establishing rigorous comparison between cultures under differing conditions. When the resource was used to evaluate the aptitude of peptones from diverse sources for the culture of lactic acid bacteria (LAB), a great variability was found, even among the results of commercial formulations with the same denomination. None of the peptones was able to maximise the growth – very active in fish peptones – and the production of the characteristic metabolites at the same time. Under these conditions, the application of the cluster analysis to kinetic parameters of proven descriptive capability becomes a useful exploratory method, which allows to decide combinations of protein sources apt to make compatible different potential purposes of LAB cultures. This way, it is possible to design factorial experiments to search optimum values for these purposes on the basis of hypothesis which avoid the selection of superfluous variables and inadequate domains.

Keywords
Logistic equation; Lactic acid bacteria; MRS media; Commercial peptones; Fish viscera peptones; Lactic acid; Nisin; Pediocin
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Mathematical tools for objective comparison of microbial cultures: Application to evaluation of 15 peptones for lactic acid bacteria productions
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 39, Issue 2, 15 April 2008, Pages 276–287
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us