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Optimization of cultivation conditions for the production of 1,3-dihydroxyacetone by Pichia membranifaciens using response surface methodology

Paper ID Volume ID Publish Year Pages File Format Full-Text
4646 236 2008 7 PDF Available
Title
Optimization of cultivation conditions for the production of 1,3-dihydroxyacetone by Pichia membranifaciens using response surface methodology
Abstract

By isolating soil samples, we obtained a novel Pichia strain (sp. ZJB-0009) capable of producing 1,3-dihydroxyacetone from glycerol. Based on the physiological, biochemical characteristics, and ITS rDNA gene sequence analysis, the strain was identified as Pichia membranifaciens and subsequently named P. membranifaciens ZJB-0009. A mathematical model was developed to investigate the influences of various fermentation parameters and to predict the optimum fermentation conditions for 1,3-dihydroxyacetone production. The maximal concentration of 1,3-dihydroxyacetone (12.91 g/L) was predicted to occur when the pH was 6.74, the incubation temperature was 29.6 °C, and the growth time was 48.8 h. A repeat fermentation of 1,3-dihydroxyacetone by P. membranifaciens ZJB-0009 was carried out in a 15-L fermenter under the optimized conditions for the verification of optimization. The maximum concentration of 1,3-dihydroxyacetone was 13.57 g/L, which was significantly higher than that obtained under unoptimized conditions.

Keywords
Pichia membranifaciens; 1,3-Dihydroxyacetone; Identification; Medium optimization; Response surface methodology; Box–Behnken design
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Optimization of cultivation conditions for the production of 1,3-dihydroxyacetone by Pichia membranifaciens using response surface methodology
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 38, Issue 3, 15 March 2008, Pages 285–291
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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Full-text PDF Download
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Any Questions? feel free to contact us