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Production and characterization of terpolyester poly(3-hydroxybutyrate-co-4-hydroxybutyrate-co-3-hydroxyhexanoate) by recombinant Aeromonas hydrophila 4AK4 harboring genes phaPCJ

Paper ID Volume ID Publish Year Pages File Format Full-Text
4659 236 2008 6 PDF Available
Title
Production and characterization of terpolyester poly(3-hydroxybutyrate-co-4-hydroxybutyrate-co-3-hydroxyhexanoate) by recombinant Aeromonas hydrophila 4AK4 harboring genes phaPCJ
Abstract

Terpolyester consisting of 3-hydroxybutyrate (3HB), 4-hydroxybutyrate (4HB) and 3-hydroxyhexanoate (3HHx) abbreviated as P(3HB-co-4HB-co-3HHx), was produced by recombinant Aeromonas hydrophila 4AK4 harboring polyhydroxyalkanoate (PHA) synthesis genes phaPCJ. The monomer content of 4HB in the terpolyesters was dependent on the concentration of 1,4-butanediol, ranging from 4.3 to 8.5 mol% in the presence of 5–25 g/l 1,4-butanediol, respectively, accompanying by a decreasing 3HHx content from 22 to 17.6 mol% and a constant 3HB content of 74 mol% during growth in lauric acid containing mineral medium. Number average molecular weights of the terpolyesters were 440,000 to 550,000, much higher than 220,000 of the copolyester consisting of 3HB and 12 mol%3HHx commonly produced by wild type A. hydrophila 4AK4. Thermal and mechanical properties of the above terpolyesters were better than those of homopolyester poly(3-hydroxybutyrate) (PHB) and its copolymers P(3HB-co-7 mol%4HB), P(3HB-co-12 mol%3HHx). Moreover, physical properties of the terpolyesters changed with variations of monomer composition. The terpolyesters were characterized using nuclear magnetic resonance (NMR), gas chromatography (GC), gel-permeation chromatography (GPC), differential scanning calorimetry (DSC), thermogravimetric analysis (TGA) and stress–strain measurements. The introduction of 4HB and 3HHx monomers into PHB enhanced the thermal stability, changed its crystallinity and flexibility compared with homopolyester PHB and their two monomer containing copolyesters.

Keywords
PHB; Aeromonas hydrophila; 1,4-Butanediol; Terpolyester; Polyhydroxyalkanoates; PHA
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Production and characterization of terpolyester poly(3-hydroxybutyrate-co-4-hydroxybutyrate-co-3-hydroxyhexanoate) by recombinant Aeromonas hydrophila 4AK4 harboring genes phaPCJ
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 38, Issue 3, 15 March 2008, Pages 384–389
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us