fulltext.study @t Gmail

Enhanced plasmid stability and production of hEGF by immobilized recombinant E. coli JM101

Paper ID Volume ID Publish Year Pages File Format Full-Text
4666 237 2006 5 PDF Available
Title
Enhanced plasmid stability and production of hEGF by immobilized recombinant E. coli JM101
Abstract

Recombinant Escherichia coli JM101 was immobilized with porous polyurethane foam (PUF) particle as supporter matrix for human epidermal growth factor (hEGF) production. Flask culture showed that cell immobilization in PUF can improve cell growth and hEGF expression. A bubble column and a three-phase fluidized bed bioreactor by self-design was further applied to produce hEGF, respectively. The results demonstrated that PUF is a feasible immobilized supporter material with good biocompatibility. Immobilization could also decrease the probability for segregational plasmid loss and overgrowth of plasmid-free cells. Cell density, plasmid stability and hEGF productivity were higher than those without the foam matrix, respectively. hEGF productivity was enhanced from 8.73 mg/l h of free-culture to 11.4 mg/l h of immobilized cultivation.

Keywords
hEGF productivity; Immobilized cultivation; Plasmid stability; Polyurethane foam; Recombinant E. coli JM101
First Page Preview
Enhanced plasmid stability and production of hEGF by immobilized recombinant E. coli JM101
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 28, Issue 3, 1 March 2006, Pages 215–219
Authors
, , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us