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Layer-by-layer self-assembly of films of nano-Au and Co(bpy)33+ for the determination of Japanese B encephalitis vaccine

Paper ID Volume ID Publish Year Pages File Format Full-Text
4668 237 2006 6 PDF Available
Title
Layer-by-layer self-assembly of films of nano-Au and Co(bpy)33+ for the determination of Japanese B encephalitis vaccine
Abstract

Nano-Au and Co(bpy)33+ in a variety of charge states were assembled layer-by-layer into films on the gold electrode modified by l-cysteine to immobilize anti-Japanese B encephalitis for the assay of Japanese B encephalitis vaccine. The electrode modification process was characterized by electrochemical impedance spectroscopy (EIS). The factors influencing the performance of the potentiometric immunosensor were studied in detail. The immunosensor exhibited fast potentiometric response and the linear range was from 8.1 × 10−8 to 3.0 × 10−6 lgpfu/ml (plaque forming unit) with a detection limit of 3.5 × 10−8 lgpfu/ml. Moreover, the studied immunosensor exhibited high sensitivity and long-term stability. The present work supplied a promising test method for biological products.

Keywords
Potentiometric immunosensor; Layer-by-layer self-assembly; Nano-Au; Japanese B encephalitis vaccine
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Layer-by-layer self-assembly of films of nano-Au and Co(bpy)33+ for the determination of Japanese B encephalitis vaccine
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 28, Issue 3, 1 March 2006, Pages 231–236
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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Full-text PDF Download
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Any Questions? feel free to contact us