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Development of new unstructured model for simultaneous saccharification and fermentation of starch to ethanol by recombinant strain

Paper ID Volume ID Publish Year Pages File Format Full-Text
4670 237 2006 13 PDF Available
Title
Development of new unstructured model for simultaneous saccharification and fermentation of starch to ethanol by recombinant strain
Abstract

The development of simultaneous saccharification and fermentation of starch to ethanol (SSFSE) by genetically modified microbial strains has been studied intensively [M.M. Altintas, B. Kirdar, Z.Ï. Önsan, K.Ö. Ülgen, Cybernetic modelling of growth and ethanol production in a recombinant Saccharomyces cerevisiae strain secreting a bifunctional fusion protein, Process Biochem. 37 (2002) 1439–1445; G. Birol, Z.Ï. Önsan, B. Kirdar, S.G. Oliver, Ethanol production and fermentation characteristics of recombinant Saccharomyces cerevisiae strains grown on starch, Enzyme Microb. Technol. 22 (1998) 672–677; F. Kobayashi, Y. Nakamura, Effect of repressor gene on stability of bioprocess with continuous conversion of starch into ethanol using recombinant yeast, Biochem. Eng. J. 18 (2004) 133–141; F. Kobayashi, Y. Nakamura, Mathematical model of direct ethanol production from starch in immobilized recombinant yeast culture, Biochem. Eng. J. 21 (2004) 93–101; M.M. Altintas, K.Ö. Ülgen, B. Kirdar, Z.Ï. Önsan, S.G. Oliver, Improvement of ethanol production from starch by recombinant yeast through manipulation of environmental factors, Enzyme Microb. Technol. 31 (2002) 640–647; K.Ö. Ülgen, B. Saygili, Z.Ï. Önsan, B. Kirdar, Bioconversion of starch into ethanol by a recombinant Saccharomyces cerevisiae strain YPG-AB, Process Biochem. 37 (2002) 1157–1168]. Saccharomyces cerevisiae YPB-G strain secretes a bifunctional fusion protein containing enzymatic activity of the B. subtilis alpha-amylase and of the Aspergillus awamori glucoamylase [M.M. Altintas, B. Kirdar, Z.Ï. Önsan, K.Ö. Ülgen, Cybernetic modelling of growth and ethanol production in a recombinant Saccharomyces cerevisiae strain secreting a bifunctional fusion protein, Process Biochem. 37 (2002) 1439–1445], and therefore is distinguished in relation to SSFSE step. In this work we have used the experimental data, presented in the paper [M.M. Altintas, B. Kirdar, Z.Ï. Önsan, K.Ö. Ülgen, Cybernetic modelling of growth and ethanol production in a recombinant Saccharomyces cerevisiae strain secreting a bifunctional fusion protein, Process Biochem. 37 (2002) 1439–1445] to develop two-hierarchic-level unstructured mathematical model describing kinetics of direct bioconversion of starch to ethanol. The first level has modeled enzymatic hydrolysis of starch to glucose by bifunctional protein and the second level includes utilization and bioconversion of glucose to ethanol by yeasts. The second level has unified the enzymatic degradation of starch, and glucose metabolization to ethanol by microorganisms. The response surface analysis was used to develop the rates models. A hybrid genetic algorithm and a decomposition approach were used in the nonlinear parameters identification procedure. The proposed model demonstrated excellent flexibility for different operational conditions of SSFSE process, and can be used successfully to describe microbial physiology of genetically modified strains.

Keywords
Recombinant yeast; Modeling; Kinetics; Ethanol; Simultaneous saccharification and fermentation
First Page Preview
Development of new unstructured model for simultaneous saccharification and fermentation of starch to ethanol by recombinant strain
Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 28, Issue 3, 1 March 2006, Pages 243–255
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering