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Equilibria and kinetics of protein transfer to and from affinity-based reverse micelles of Span 85 modified with Cibacron Blue F-3GA

Paper ID Volume ID Publish Year Pages File Format Full-Text
4674 237 2006 8 PDF Available
Title
Equilibria and kinetics of protein transfer to and from affinity-based reverse micelles of Span 85 modified with Cibacron Blue F-3GA
Abstract

Sorbitan trioleate was modified with Cibacron Blue F-3GA (CB) to create an affinity surfactant and to form affinity-based reverse micelles in n-hexane. The partitioning equilibria and the extraction kinetics of lysozyme and bovine serum albumin (BSA) were then examined. The solubilization capacity of the reverse micellar system for lysozyme increased linearly with increasing the CB concentration from 0.1 to 0.5 mmol L−1. In contrast, the capacity for BSA at 0.5 mmol L−1 of coupled CB was only about one-fifth that for lysozyme. It indicates a strong steric hindrance effect of the micelles for the high molecular mass protein. The overall volumetric mass transfer coefficient of lysozyme in the forward extraction increased from 0.43 × 10−3 to 1.25 × 10−3 s−1 with increasing CB concentration from 0.1 to 0.5 mmol L−1. Due to the high molecular mass of BSA, its volumetric mass transfer coefficient in the forward extraction was only one-sixth that of lysozyme. The ratio of the coefficient in the back extraction to that in the forward extraction was less than 0.03, much lower than those in other micellar systems. It indicates that the interfacial resistance in this system was severer than in others.

Keywords
Affinity; Reverse micelles; Nonionic surfactant; Protein; Equilibrium; Extraction kinetics
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Equilibria and kinetics of protein transfer to and from affinity-based reverse micelles of Span 85 modified with Cibacron Blue F-3GA
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 28, Issue 3, 1 March 2006, Pages 281–288
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us