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Substrate specificity, regioselectivity and hydrolytic activity of lipases activated from Geotrichum sp.

Paper ID Volume ID Publish Year Pages File Format Full-Text
4685 238 2007 8 PDF Available
Title
Substrate specificity, regioselectivity and hydrolytic activity of lipases activated from Geotrichum sp.
Abstract

Substrate specificity (typoselectivity), regioselectivity and hydrolytic activity of induced lipases from three strains (4012, 4013, 4166) of Geotrichum candidum and that of Geotrichum ludwigii (48) were investigated. The lipases were induced in two types of culture media, of which the medium containing peptone as nitrogen source was proved to give better results. Olive oil was employed as inductor for the lipase activity. Activated lipases represented mostly extracelullar lipases, which penetrated through cellular membrane into medium. The activity of cell-bound lipase was also determined. Most of lipases belong to the group of specific lipases able to hydrolyse ester bonds in the positions sn-1 and sn-3 ester of triacylglycerols (1,3-selective lipases) and display specificity to saturated fatty acids. All activated lipases from Geotrichum sp., extracellular and cell-bound, were used as biocatalyst in the blackcurrant oil hydrolysis.

Keywords
Biocatalysis; Enzyme activity; Gas chromatography; Lipase; Blackcurrant oil; Geotrichum sp
First Page Preview
Substrate specificity, regioselectivity and hydrolytic activity of lipases activated from Geotrichum sp.
Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 34, Issue 3, June 2007, Pages 209–216
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering